Geochemistry: Exploration, Environment, Analysis; May 2002; v. 2; no. 2;
p. 151-156; DOI: 10.1144/1467-787302-018
© 2002 Geological Society of London
Copper tolerance testing in populations of Mimulus luteus var. variegatus exposed and non-exposed to copper mine pollution
R. Ginocchio1,
I. Toro1,
D. Schnepf2 and
M. R. Macnair3
1 Departamento
de Ecología, Pontificia Universidad Católica de Chile,
Alameda 340, Santiago,
Chile(e-mail:
ERGINOCC{at}GENES.BIO.PUC.CL)
2 Agricultural
University, Vienna,
Austria
3 Department of
Biological Sciences, Hatherly Laboratories, Prince of Wales Road,
Exeter EX4 4PS,
UK
Several
plant species have evolved metal tolerance as a response to increasing
metal concentration in soils due to human activities. A well-described
example is the evolution of copper-tolerant ecotypes of the monkey
flower (Mimulus guttatus) in Copperopolis, California. Although
copper mine activities have been intense in some areas of the Andes
Range in central Chile, metal-tolerant ecotypes have not been reported
for any native or endemic plant species. Copper tolerance of two
populations of Mimulus luteus var. variegatus from
pristine basins (Río Cipreses and Las Cayanas) and one
population from a highly polluted copper mine area (Sewell) were tested
to determine their copper tolerance. Tolerance was tested on cuttings
collected in the field from adult plants (Sewell population) and on
cuttings from plants cultivated from seeds in the laboratory
(Río Cipreses, Las Cayanas and Sewell). All cuttings were grown
under increasing concentrations of copper (0, 0.5 and
2.0 mg l1) in calcium
nitrate (0.5 g l1).
Results indicated that root number and length were strongly inhibited
in M. luteus var. variegatus from Río Cipreses and
Las Cayanas, but tolerant individuals were found in the Sewell
population. The degree of copper tolerance of M. luteus var.
variegatus from Sewell was, however, lower than that of
individuals of Mimulus guttatus from
Copperopolis.
KEYWORDS: metal soil
pollution, smelters, pseudometallophytes, Chile
Copyright © 2009 by Geological Society of London